What we get first from our DNA sequencers is the raw data. How do we manipulate results before sending them out? Raw data (data before analysis by the basecaller algorithm) are data as they are recorded by the sequencer: phd.1 you can easily open and examine in any text editor of your choice.Įlectropherogram (data after analysis) shows a sequence of peaks in four colors, each color represents the base called for that peak and there is a textual version of recorded sequence visible: phd.1 file (Phred file) is a simple text file containing bases with quality values for each base.īoth. seq file is a simple sequence text file in FASTA format. ab1 for a thorough data interpretation (including Raw data) instead. The electropherogram is also saved and provided as a. There are various basecalling algorithms available and we always try and choose the one which gives best results. You always receive it processed from us, where processed means examined by a basecalling program. ab1 file contains the DNA sequence electropherogram as well as raw data and some other information. Results of DNA sequencing are provided in three data files –.
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